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jawsii murine dendritic cells  (ATCC)
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ATCC jawsii murine dendritic cells
CALR-treated macrophages <t>stimulate</t> <t>dendritic</t> cells (A) Media from CALR-stimulated macrophages was added to cultures of dendritic cells. Either CALR or untransformed bacterial lysate was added to RAW264.7 macrophages for 24 h ( n = 3). The conditioned media were removed and mixed 30:70 with fresh media and added to cultures of <t>JAWSII</t> dendritic cells. For a second set of macrophage-free cultures, CALR or untransformed bacterial lysates were added to plates without macrophages. After 24 h, these media were mixed with fresh media and transferred to cultures of JAWSII dendritic cells, using the same procedure as conditioned cultures. (B) Media taken from CALR-treated macrophages ( Cond. CALR ) significantly increased the expression CD80 by dendritic cells compared to bacterial controls ( Cond. BC , p < 0.0001). Media from CALR-treated macrophages also increased CD80 expression compared to macrophage-free ( MΦ-free CALR ) media ( p = 0.001). There was no difference between either bacterial controls ( Cond. BC ) or macrophage-free media and dendritic cells grown in fresh media ( Untreated ). (C) CALR-conditioned media increased expression of CD86 by dendritic cells compared to media conditioned by control bacterial lysate ( p < 0.0001). Macrophage-free CALR media also increased CD86 expression compared to macrophage-free with bacterial control lysate ( MΦ-free BC , p < 0.0001). (D) Direct addition of CALR lysate to JAWSII dendritic cells increased CD80 expression ( p = 0.004) compared to untreated controls. (E) Direct addition of CALR lysate significantly increased CD86 expression compared to both bacterial controls ( BC , p < 0.000) and untreated controls ( p < 0.000). Data are represented as mean ± SEM. The statistical comparisons in (B–E) are ANOVA followed by Tukey’s method. Asterisks indicate significance: ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
Jawsii Murine Dendritic Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine bone marrow dendritic cells dc jawsii cell line  (ATCC)
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ATCC murine bone marrow dendritic cells dc jawsii cell line
CALR-treated macrophages <t>stimulate</t> <t>dendritic</t> cells (A) Media from CALR-stimulated macrophages was added to cultures of dendritic cells. Either CALR or untransformed bacterial lysate was added to RAW264.7 macrophages for 24 h ( n = 3). The conditioned media were removed and mixed 30:70 with fresh media and added to cultures of <t>JAWSII</t> dendritic cells. For a second set of macrophage-free cultures, CALR or untransformed bacterial lysates were added to plates without macrophages. After 24 h, these media were mixed with fresh media and transferred to cultures of JAWSII dendritic cells, using the same procedure as conditioned cultures. (B) Media taken from CALR-treated macrophages ( Cond. CALR ) significantly increased the expression CD80 by dendritic cells compared to bacterial controls ( Cond. BC , p < 0.0001). Media from CALR-treated macrophages also increased CD80 expression compared to macrophage-free ( MΦ-free CALR ) media ( p = 0.001). There was no difference between either bacterial controls ( Cond. BC ) or macrophage-free media and dendritic cells grown in fresh media ( Untreated ). (C) CALR-conditioned media increased expression of CD86 by dendritic cells compared to media conditioned by control bacterial lysate ( p < 0.0001). Macrophage-free CALR media also increased CD86 expression compared to macrophage-free with bacterial control lysate ( MΦ-free BC , p < 0.0001). (D) Direct addition of CALR lysate to JAWSII dendritic cells increased CD80 expression ( p = 0.004) compared to untreated controls. (E) Direct addition of CALR lysate significantly increased CD86 expression compared to both bacterial controls ( BC , p < 0.000) and untreated controls ( p < 0.000). Data are represented as mean ± SEM. The statistical comparisons in (B–E) are ANOVA followed by Tukey’s method. Asterisks indicate significance: ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
Murine Bone Marrow Dendritic Cells Dc Jawsii Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell separation kit  (Miltenyi Biotec)
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Miltenyi Biotec cell separation kit
CALR-treated macrophages <t>stimulate</t> <t>dendritic</t> cells (A) Media from CALR-stimulated macrophages was added to cultures of dendritic cells. Either CALR or untransformed bacterial lysate was added to RAW264.7 macrophages for 24 h ( n = 3). The conditioned media were removed and mixed 30:70 with fresh media and added to cultures of <t>JAWSII</t> dendritic cells. For a second set of macrophage-free cultures, CALR or untransformed bacterial lysates were added to plates without macrophages. After 24 h, these media were mixed with fresh media and transferred to cultures of JAWSII dendritic cells, using the same procedure as conditioned cultures. (B) Media taken from CALR-treated macrophages ( Cond. CALR ) significantly increased the expression CD80 by dendritic cells compared to bacterial controls ( Cond. BC , p < 0.0001). Media from CALR-treated macrophages also increased CD80 expression compared to macrophage-free ( MΦ-free CALR ) media ( p = 0.001). There was no difference between either bacterial controls ( Cond. BC ) or macrophage-free media and dendritic cells grown in fresh media ( Untreated ). (C) CALR-conditioned media increased expression of CD86 by dendritic cells compared to media conditioned by control bacterial lysate ( p < 0.0001). Macrophage-free CALR media also increased CD86 expression compared to macrophage-free with bacterial control lysate ( MΦ-free BC , p < 0.0001). (D) Direct addition of CALR lysate to JAWSII dendritic cells increased CD80 expression ( p = 0.004) compared to untreated controls. (E) Direct addition of CALR lysate significantly increased CD86 expression compared to both bacterial controls ( BC , p < 0.000) and untreated controls ( p < 0.000). Data are represented as mean ± SEM. The statistical comparisons in (B–E) are ANOVA followed by Tukey’s method. Asterisks indicate significance: ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
Cell Separation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jawsii mouse bone marrow derived dendritic cells  (ATCC)
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ATCC jawsii mouse bone marrow derived dendritic cells
CALR-treated macrophages <t>stimulate</t> <t>dendritic</t> cells (A) Media from CALR-stimulated macrophages was added to cultures of dendritic cells. Either CALR or untransformed bacterial lysate was added to RAW264.7 macrophages for 24 h ( n = 3). The conditioned media were removed and mixed 30:70 with fresh media and added to cultures of <t>JAWSII</t> dendritic cells. For a second set of macrophage-free cultures, CALR or untransformed bacterial lysates were added to plates without macrophages. After 24 h, these media were mixed with fresh media and transferred to cultures of JAWSII dendritic cells, using the same procedure as conditioned cultures. (B) Media taken from CALR-treated macrophages ( Cond. CALR ) significantly increased the expression CD80 by dendritic cells compared to bacterial controls ( Cond. BC , p < 0.0001). Media from CALR-treated macrophages also increased CD80 expression compared to macrophage-free ( MΦ-free CALR ) media ( p = 0.001). There was no difference between either bacterial controls ( Cond. BC ) or macrophage-free media and dendritic cells grown in fresh media ( Untreated ). (C) CALR-conditioned media increased expression of CD86 by dendritic cells compared to media conditioned by control bacterial lysate ( p < 0.0001). Macrophage-free CALR media also increased CD86 expression compared to macrophage-free with bacterial control lysate ( MΦ-free BC , p < 0.0001). (D) Direct addition of CALR lysate to JAWSII dendritic cells increased CD80 expression ( p = 0.004) compared to untreated controls. (E) Direct addition of CALR lysate significantly increased CD86 expression compared to both bacterial controls ( BC , p < 0.000) and untreated controls ( p < 0.000). Data are represented as mean ± SEM. The statistical comparisons in (B–E) are ANOVA followed by Tukey’s method. Asterisks indicate significance: ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
Jawsii Mouse Bone Marrow Derived Dendritic Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human 130 097 240 miltenyi biotec  (Miltenyi Biotec)
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CALR-treated macrophages <t>stimulate</t> <t>dendritic</t> cells (A) Media from CALR-stimulated macrophages was added to cultures of dendritic cells. Either CALR or untransformed bacterial lysate was added to RAW264.7 macrophages for 24 h ( n = 3). The conditioned media were removed and mixed 30:70 with fresh media and added to cultures of <t>JAWSII</t> dendritic cells. For a second set of macrophage-free cultures, CALR or untransformed bacterial lysates were added to plates without macrophages. After 24 h, these media were mixed with fresh media and transferred to cultures of JAWSII dendritic cells, using the same procedure as conditioned cultures. (B) Media taken from CALR-treated macrophages ( Cond. CALR ) significantly increased the expression CD80 by dendritic cells compared to bacterial controls ( Cond. BC , p < 0.0001). Media from CALR-treated macrophages also increased CD80 expression compared to macrophage-free ( MΦ-free CALR ) media ( p = 0.001). There was no difference between either bacterial controls ( Cond. BC ) or macrophage-free media and dendritic cells grown in fresh media ( Untreated ). (C) CALR-conditioned media increased expression of CD86 by dendritic cells compared to media conditioned by control bacterial lysate ( p < 0.0001). Macrophage-free CALR media also increased CD86 expression compared to macrophage-free with bacterial control lysate ( MΦ-free BC , p < 0.0001). (D) Direct addition of CALR lysate to JAWSII dendritic cells increased CD80 expression ( p = 0.004) compared to untreated controls. (E) Direct addition of CALR lysate significantly increased CD86 expression compared to both bacterial controls ( BC , p < 0.000) and untreated controls ( p < 0.000). Data are represented as mean ± SEM. The statistical comparisons in (B–E) are ANOVA followed by Tukey’s method. Asterisks indicate significance: ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
Human 130 097 240 Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human diamond plasmacytoid dendritic cell isolation kit ii  (Miltenyi Biotec)
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Miltenyi Biotec human diamond plasmacytoid dendritic cell isolation kit ii
CALR-treated macrophages <t>stimulate</t> <t>dendritic</t> cells (A) Media from CALR-stimulated macrophages was added to cultures of dendritic cells. Either CALR or untransformed bacterial lysate was added to RAW264.7 macrophages for 24 h ( n = 3). The conditioned media were removed and mixed 30:70 with fresh media and added to cultures of <t>JAWSII</t> dendritic cells. For a second set of macrophage-free cultures, CALR or untransformed bacterial lysates were added to plates without macrophages. After 24 h, these media were mixed with fresh media and transferred to cultures of JAWSII dendritic cells, using the same procedure as conditioned cultures. (B) Media taken from CALR-treated macrophages ( Cond. CALR ) significantly increased the expression CD80 by dendritic cells compared to bacterial controls ( Cond. BC , p < 0.0001). Media from CALR-treated macrophages also increased CD80 expression compared to macrophage-free ( MΦ-free CALR ) media ( p = 0.001). There was no difference between either bacterial controls ( Cond. BC ) or macrophage-free media and dendritic cells grown in fresh media ( Untreated ). (C) CALR-conditioned media increased expression of CD86 by dendritic cells compared to media conditioned by control bacterial lysate ( p < 0.0001). Macrophage-free CALR media also increased CD86 expression compared to macrophage-free with bacterial control lysate ( MΦ-free BC , p < 0.0001). (D) Direct addition of CALR lysate to JAWSII dendritic cells increased CD80 expression ( p = 0.004) compared to untreated controls. (E) Direct addition of CALR lysate significantly increased CD86 expression compared to both bacterial controls ( BC , p < 0.000) and untreated controls ( p < 0.000). Data are represented as mean ± SEM. The statistical comparisons in (B–E) are ANOVA followed by Tukey’s method. Asterisks indicate significance: ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
Human Diamond Plasmacytoid Dendritic Cell Isolation Kit Ii, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CALR-treated macrophages <t>stimulate</t> <t>dendritic</t> cells (A) Media from CALR-stimulated macrophages was added to cultures of dendritic cells. Either CALR or untransformed bacterial lysate was added to RAW264.7 macrophages for 24 h ( n = 3). The conditioned media were removed and mixed 30:70 with fresh media and added to cultures of <t>JAWSII</t> dendritic cells. For a second set of macrophage-free cultures, CALR or untransformed bacterial lysates were added to plates without macrophages. After 24 h, these media were mixed with fresh media and transferred to cultures of JAWSII dendritic cells, using the same procedure as conditioned cultures. (B) Media taken from CALR-treated macrophages ( Cond. CALR ) significantly increased the expression CD80 by dendritic cells compared to bacterial controls ( Cond. BC , p < 0.0001). Media from CALR-treated macrophages also increased CD80 expression compared to macrophage-free ( MΦ-free CALR ) media ( p = 0.001). There was no difference between either bacterial controls ( Cond. BC ) or macrophage-free media and dendritic cells grown in fresh media ( Untreated ). (C) CALR-conditioned media increased expression of CD86 by dendritic cells compared to media conditioned by control bacterial lysate ( p < 0.0001). Macrophage-free CALR media also increased CD86 expression compared to macrophage-free with bacterial control lysate ( MΦ-free BC , p < 0.0001). (D) Direct addition of CALR lysate to JAWSII dendritic cells increased CD80 expression ( p = 0.004) compared to untreated controls. (E) Direct addition of CALR lysate significantly increased CD86 expression compared to both bacterial controls ( BC , p < 0.000) and untreated controls ( p < 0.000). Data are represented as mean ± SEM. The statistical comparisons in (B–E) are ANOVA followed by Tukey’s method. Asterisks indicate significance: ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
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CALR-treated macrophages stimulate dendritic cells (A) Media from CALR-stimulated macrophages was added to cultures of dendritic cells. Either CALR or untransformed bacterial lysate was added to RAW264.7 macrophages for 24 h ( n = 3). The conditioned media were removed and mixed 30:70 with fresh media and added to cultures of JAWSII dendritic cells. For a second set of macrophage-free cultures, CALR or untransformed bacterial lysates were added to plates without macrophages. After 24 h, these media were mixed with fresh media and transferred to cultures of JAWSII dendritic cells, using the same procedure as conditioned cultures. (B) Media taken from CALR-treated macrophages ( Cond. CALR ) significantly increased the expression CD80 by dendritic cells compared to bacterial controls ( Cond. BC , p < 0.0001). Media from CALR-treated macrophages also increased CD80 expression compared to macrophage-free ( MΦ-free CALR ) media ( p = 0.001). There was no difference between either bacterial controls ( Cond. BC ) or macrophage-free media and dendritic cells grown in fresh media ( Untreated ). (C) CALR-conditioned media increased expression of CD86 by dendritic cells compared to media conditioned by control bacterial lysate ( p < 0.0001). Macrophage-free CALR media also increased CD86 expression compared to macrophage-free with bacterial control lysate ( MΦ-free BC , p < 0.0001). (D) Direct addition of CALR lysate to JAWSII dendritic cells increased CD80 expression ( p = 0.004) compared to untreated controls. (E) Direct addition of CALR lysate significantly increased CD86 expression compared to both bacterial controls ( BC , p < 0.000) and untreated controls ( p < 0.000). Data are represented as mean ± SEM. The statistical comparisons in (B–E) are ANOVA followed by Tukey’s method. Asterisks indicate significance: ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Journal: Molecular Therapy Oncology

Article Title: Recombinant CALR polarizes and activates macrophages in tumors

doi: 10.1016/j.omton.2025.201121

Figure Lengend Snippet: CALR-treated macrophages stimulate dendritic cells (A) Media from CALR-stimulated macrophages was added to cultures of dendritic cells. Either CALR or untransformed bacterial lysate was added to RAW264.7 macrophages for 24 h ( n = 3). The conditioned media were removed and mixed 30:70 with fresh media and added to cultures of JAWSII dendritic cells. For a second set of macrophage-free cultures, CALR or untransformed bacterial lysates were added to plates without macrophages. After 24 h, these media were mixed with fresh media and transferred to cultures of JAWSII dendritic cells, using the same procedure as conditioned cultures. (B) Media taken from CALR-treated macrophages ( Cond. CALR ) significantly increased the expression CD80 by dendritic cells compared to bacterial controls ( Cond. BC , p < 0.0001). Media from CALR-treated macrophages also increased CD80 expression compared to macrophage-free ( MΦ-free CALR ) media ( p = 0.001). There was no difference between either bacterial controls ( Cond. BC ) or macrophage-free media and dendritic cells grown in fresh media ( Untreated ). (C) CALR-conditioned media increased expression of CD86 by dendritic cells compared to media conditioned by control bacterial lysate ( p < 0.0001). Macrophage-free CALR media also increased CD86 expression compared to macrophage-free with bacterial control lysate ( MΦ-free BC , p < 0.0001). (D) Direct addition of CALR lysate to JAWSII dendritic cells increased CD80 expression ( p = 0.004) compared to untreated controls. (E) Direct addition of CALR lysate significantly increased CD86 expression compared to both bacterial controls ( BC , p < 0.000) and untreated controls ( p < 0.000). Data are represented as mean ± SEM. The statistical comparisons in (B–E) are ANOVA followed by Tukey’s method. Asterisks indicate significance: ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Article Snippet: JAWSII murine dendritic cells and CT26 murine colon carcinoma cells were obtained from ATCC, confirmed by STR profiling by Charles River Research Animal Diagnostic Services, and passaged for fewer than 6 months.

Techniques: Expressing, Control